Silage Formulation Based on Tithonia diversifolia and Panicum maximum: In Vitro Fermentation Evaluation for Tropical Feeding Systems

Authors

  • Dwi Ananta Department of Livestock Production Technology, State Agricultural Polytechnic of Payakumbuh
  • Nadia Rahma Department of Livestock Production Technology, State Agricultural Polytechnic of Payakumbuh
  • Fadilla Meidita Department of Livestock Production Technology, State Agricultural Polytechnic of Payakumbuh
  • Yudha Endra Pratama Faculty of Animal Science, Universitas Andalas

DOI:

https://doi.org/10.25077/alive.v2.n2.p144-151.2025

Keywords:

in vitro fermentation, panicum maximum, silage, tithonia diversifolia

Abstract

Ensuring year-round availability of high-quality forage is critical for improving the productivity of ruminant livestock, particularly under tropical climatic conditions where seasonal fluctuations frequently disrupt feed supply. This study evaluated the fermentative quality of silage produced from varying ratios of Tithonia diversifolia and Panicum maximum using an in vitro rumen fermentation system. A Completely Randomized Design (CRD) was applied with five treatment groups (T0 = 0% Tithonia, T1 = 25%, T2 = 50%, T3 = 75%, and T4 = 100%) and three replicates each. After a 21-day ensiling period, samples were assessed for pH, total gas production, ammonia nitrogen (NH₃-N), and volatile fatty acids (VFA) using fresh rumen fluid from slaughtered cattle as the inoculum. Results revealed that increasing the proportion of T. diversifolia enhanced fermentative activity, as indicated by higher total gas and VFA production, and lower pH values. Treatment T3 (75% Tithonia) showed the most favorable fermentative profile, with significantly higher gas and VFA yields and NH₃-N concentrations within the optimal range for microbial protein synthesis. Conversely, T4 (100% Tithonia) exhibited signs of fermentative inhibition, likely due to anti-nutritional factors such as tannins and saponins. In contrast, T0 (100% Panicum) demonstrated limited fermentability due to its high fiber and low soluble carbohydrate content. These findings suggest that a 75:25 ratio of Tithonia diversifolia to Panicum maximum optimizes rumen fermentation and may serve as a promising strategy for improving silage quality in tropical forage systems.

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References

Addawiyyah, N. R., Ayuningsih, B., Budiman, A., & Hernaman, I. (2021). Produksi gas pada ransum domba berbasis rumput gajah cv Mott dan leguminosa pohon. Jurnal Sumber Daya Hewan, 2(2). DOI: https://doi.org/10.24198/jsdh.v2i2.35069

Afdal, M., & Yurleni. (2015). Pengaruh modifikasi inokulum feses sebagai pengganti cairan rumen pada teknik in vitro: Estimasi kecernaan NDF, ADF, dan protein kasar rumput lapangan. Jurnal Ilmu-Ilmu Peternakan, 18(2), 83–90. DOI: https://doi.org/10.22437/jiiip.v18i2.2677

Arief, A., Elihasridas., Somen, S., Roza, E., Pazla, R., & Rizqan. (2018). Production and quality of Etawa raw milk using palm oil industry waste and paitan plants as an early feed. Pakistan Journal of Nutrition, 17(8), 399–404. https://doi.org/10.3923/pjn.2018.399.404 DOI: https://doi.org/10.3923/pjn.2018.399.404

Baga, A. S., Lazarus, E. J. L., Hilakore, M. A., & Lawa, E. D. W. (2023). Pengaruh penggunaan sumber karbohidrat mudah larut sebagai aditif terhadap komposisi fraksi serat silase isi rumen sapi. Stock Peternakan, 5(2). https://ojs.universitasmuarabungo.ac.id/index.php/Sptr/index DOI: https://doi.org/10.33474/rekasatwa.v5i2.20815

Bakhtiar, A. Y., Sutrisno, & Sunarso. (2013). Pengaruh proteksi protein bungkil kelapa sawit dengan tanin terhadap fermentabilitasnya secara in vitro. Animal Agriculture Journal, 2(1), 232–239. https://ejournal-s1.undip.ac.id/index.php/aaj

Dhalika, T., Budiman, A., & Mansyur. (2015). Kualitas silase rumput Benggala (Panicum maximum) pada berbagai taraf penambahan bahan aditif ekstrak cairan asam laktat produk fermentasi anaerob batang pisang. Jurnal Peternakan Indonesia, 17(1), 77–84. DOI: https://doi.org/10.25077/jpi.17.1.77-82.2015

Dhalika, T., Budiman, A., & Tarmidi, A. R. (2021). Pengaruh penambahan molases pada proses ensilase terhadap kualitas silase jerami ubi jalar (Ipomoea batatas). Jurnal Ilmu Ternak, 21(1), 33–39. https://doi.org/10.24198/jit.v21i1.33105 DOI: https://doi.org/10.24198/jit.v21i1.33105

Fitria, S. R., Darwin, & Putra, B. S. (2024). Fermentasi berbagai jenis pakan silase pada cairan rumen sapi. Jurnal Ilmiah Mahasiswa Pertanian, 9(1). DOI: https://doi.org/10.17969/jimfp.v9i1.29059

Jamarun, N., Ikhlas, Z., Zain, M., Negara, W., Pazla, R., & Yanti, G. (2024). Feed wafers from fermented sugarcane tops and Tithonia diversifolia with added tapioca starch: Effects on physical quality and in-vitro parameters for ruminant feed. Open Veterinary Journal, 14(12), 3599-3613. https://doi.org/10.5455/OVJ.2024.v14.i12.41 DOI: https://doi.org/10.5455/OVJ.2024.v14.i12.41

Jamarun, N., Pazla, R., Zain, M., & Arief. (2019). Comparison of in vitro digestibility and rumen fluid characteristic between the tithonia (Tithonia diversifolia) with elephant grass (Pennisetum purpureum). IOP Conference Series: Earth and Environmental Science, 287, 012019. https://doi.org/10.1088/1755-1315/287/1/012019 DOI: https://doi.org/10.1088/1755-1315/287/1/012019

Jamarun, N., Pazla, R., Zain, M., & Arief. (2020). Milk quality of etawa crossbred dairy goat fed combination of fermented oil palm fronds, tithonia (Tithonia diversifolia) and elephant grass (Pennisetum purpureum). Journal of Physics: Conference Series, 1469(1), 012004. https://doi.org/10.1088/1742-6596/1469/1/012004 DOI: https://doi.org/10.1088/1742-6596/1469/1/012004

Landupari, M., Foekh, A. H. B., & Utami, K. B. (2020). Pembuatan silase rumput gajah odot (Pennisetum purpureum cv. Mott) dengan penambahan berbagai dosis molasses. Jurnal Peternakan Indonesia, 22(2), 249–253. https://doi.org/10.25077/jpi.22.2.249-253.2020 DOI: https://doi.org/10.25077/jpi.22.2.249-253.2020

Mariani, N. P., & Suryani, N. N. (2016). Kecernaan dan produk fermentasi rumen (in vitro) ransum sapi Bali induk dengan level energi berbeda. Majalah Ilmiah Peternakan, 19(3). DOI: https://doi.org/10.24843/MIP.2016.v19.i03.p01

Muchlis, A., Sema, Syamsu, J. A., & Asmuddin. (2023). Article review: Teknologi pengolahan pakan hijauan untuk ternak sapi di daerah tropis. Jurnal Ilmu dan Teknologi Peternakan Terpadu, 1(1), 145–152. https://doi.org/10.56326/jitpu.v3i1.2528 DOI: https://doi.org/10.56326/jitpu.v3i1.2528

Nurwildan, S. N., Susilawati, I., & Mutaqin, B. K. (2022). Kandungan VFA dan NH₃ pada rumen domba secara in vitromenggunakan beberapa rumput yang ditanam di Desa Ujungjaya, Sumedang. Jurnal Sumber Daya Hewan, 3(1).

Pazla, R., Jamarun, N., Agustin, F., Zain, M., & Cahyani, N. O. (2021b). In vitro nutrient digestibility, volatile fatty acids, and gas production of fermented palm fronds combined with tithonia (Tithonia diversifolia) and elephant grass (Pennisetum purpureum). IOP Conference Series: Earth and Environmental Science, 888, 012067. https://doi.org/10.1088/1755-1315/888/1/012067 DOI: https://doi.org/10.1088/1755-1315/888/1/012067

Pazla, R., Jamarun, N., Zain, M., & Arief. (2018). Microbial protein synthesis and in vitro fermentability of fermented oil palm fronds by Phanerochaete chrysosporium in combination with tithonia (Tithonia diversifolia) and elephant grass (Pennisetum purpureum). Pakistan Journal of Nutrition, 17(10), 462–470. https://doi.org/10.3923/pjn.2018.462.470 DOI: https://doi.org/10.3923/pjn.2018.462.470

Pazla, R., Jamarun, N., Zain, M., Yanti, G., & Chandra, R. H. (2021a). Quality evaluation of tithonia (Tithonia diversifolia) with fermentation using Lactobacillus plantarum and Aspergillus ficuum at different incubation times. Biodiversitas Journal of Biological Divers, 22(9), 3936–3942. https://doi.org/10.13057/biodiv/d220940 DOI: https://doi.org/10.13057/biodiv/d220940

Putri, E. M., Pazla, R., Jamarun, N., Agustin, F., Yanti, G., Ikhlas, Z., Arief, A., Negara, W., Khan, F. E., Syrachman, M., Darmawan, W. A., Akhadiarto, S., Herdis, H., Priyatno, T. P., & Lestari, P. (2024). Optimizing ruminant feed efficiency: The synergetic effects of BMR sorghum and Tithonia diversifolia on nutrient digestibility, rumen function, and methane mitigation. Journal of Animal Behaviour and Biometeorology, 12(3), e2024022. https://doi.org/10.31893/jabb.2024022 DOI: https://doi.org/10.31893/jabb.2024022

Sirait, J., & Simanihuruk, K. (2021). Pemanfaatan Tithonia diversifolia sebagai pakan ruminansia. WARTAZOA, 31(3), 137–146. https://doi.org/10.14334/wartazoa.v31i2.2876 DOI: https://doi.org/10.14334/wartazoa.v31i3.2876

Suharti, S., Aliyah, D. N., & Suryahadi. (2018). Karakteristik fermentasi rumen in vitro dengan penambahan sabun kalsium minyak nabati pada buffer yang berbeda. Jurnal Ilmu Nutrisi dan Teknologi Pakan, 16(3), 56–64. https://doi.org/10.29244/jintp.16.3.56-64 DOI: https://doi.org/10.29244/jintp.16.3.56-64

Wijayanti, E., Wahyono, F., & Surono. (2012). Kecernaan nutrien dan fermentabilitas pakan komplit dengan level ampas tebu yang berbeda secara in vitro. Animal Agricultural Journal, 1(1), 167–179. https://ejournal-s1.undip.ac.id/index.php/aaj

Yogianto, Sudarman, A., Jayanegara, A., & Wina, E. (2014). Kajian in vitro reduksi emisi gas metana melalui penambahan ekstrak tanin dan saponin dalam pakan dengan proporsi hijauan berbeda [Skripsi/Tesis tidak dipublikasikan]. IPB Scientific Repository. https://repository.ipb.ac.id/handle/123456789/71198

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Published

2025-07-30

How to Cite

Ananta, D., Rahma, N., Meidita, F., & Pratama, Y. E. (2025). Silage Formulation Based on Tithonia diversifolia and Panicum maximum: In Vitro Fermentation Evaluation for Tropical Feeding Systems. Andalasian Livestock, 2(2), 144–151. https://doi.org/10.25077/alive.v2.n2.p144-151.2025

Issue

Vol. 2 No. 2 (2025): ALive

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Copyright (c) 2025 Dwi Ananta, Nadia Rahma, Fadilla Meidita, Yudha Endra Pratama

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