Interaction of Palm Fatty Acid Distillate (PFAD) and High-Tannin Mangrove Fruit Extract (Sonneratia alba) on the In Vitro Digestibility of Fiber Fractions in Dairy Cattle Feed
DOI:
https://doi.org/10.25077/alive.v2.n2.p178-184.2025Keywords:
Dairy cattle, fiber digestibility, in vitro, PFAD, Sonneratia alba, tanninAbstract
This study aimed to evaluate the interaction between Palm Fatty Acid Distillate (PFAD) and high-tannin mangrove fruit extract (Sonneratia alba) on the in vitro digestibility of dairy cattle feed fiber fractions. The experimental design used a completely randomized factorial 3×4 pattern with three replicates. The first factor was the PFAD level (0%, 3%, 6%) and the second factor was the mangrove fruit extract dose (0%, 0.5%, 1%, 2%). Fermentation was carried out using the Tilley and Terry (1963) rumen stage method using fresh goat rumen fluid. The parameters observed included the digestibility of neutral detergent fiber (NDF), acid detergent fiber (ADF), hemicellulose, and cellulose. The results showed that PFAD, mangrove fruit extract, and their interaction had a significant effect (P<0.05) on the digestibility of NDF and cellulose, but did not have a significant effect on ADF and hemicellulose. The combination of 3% PFAD and 0.5–1% extract provided the highest digestibility. Increasing PFAD and tannin reduced digestibility due to the fat coating effect and the formation of tannin complexes with structural carbohydrates. The combination of PFAD and mangrove tannin can be optimized as a functional feed additive based on local resources that improves rumen fermentation efficiency without reducing fiber digestibility.
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Aboagye, I. A., & Beauchemin, K. A. (2019). Potential of molecular weight and structure of tannins to reduce methane emissions from ruminants: A review. Animals, 9(11), 856. https://doi.org/10.3390/ani9110856 DOI: https://doi.org/10.3390/ani9110856
Ardani, L.R., Zain, M., Pazla, R., Utami, Y., Sari, R.R., Hadiwijaya, M.T., Putri, E.M., & Makmur, M. (2024). Optimization of Indigofera zollingeriana and gambier (Uncaria gambir) supplementation on feed consumption, digestibility, methane production and lactation performance of Etawa crossbreed goats. International Journal of Veterinary Science, 13(5), 537–544. https://doi.org/10.47278/journal.ijvs/2024.121
Besharati, M., Maggiolino, A., Palangi, V., Kaya, A., Jabbar, M., Eseceli, H., De Palo, P., & Lorenzo, M. (2022). Tannin in ruminant nutrition: A review. Molecules, 27(23), 8273. https://doi.org/10.3390/molecules27238273 DOI: https://doi.org/10.3390/molecules27238273
Carreño, D., Hervás, G., Toral, P.G., & Frutos, P. (2015). Ability of different types and doses of tannin extracts to modulate in vitro ruminal biohydrogenation in sheep. Animal Feed Science and Technology, 202, 42–51. https://doi.org/10.1016/j.anifeedsci.2015.02.003 DOI: https://doi.org/10.1016/j.anifeedsci.2015.02.003
Chen, L., Bao, X., Guo, G., Huo, W., Xu, Q., Wang, C., Li, Q., & Liu, Q. (2021). Effects of hydrolysable tannin with or without condensed tannin on alfalfa silage fermentation characteristics and in vitro ruminal methane production, fermentation patterns, and microbiota. Animals, 11(7), 1967. https://doi.org/10.3390/ani11071967 DOI: https://doi.org/10.3390/ani11071967
Dalby, F.R., Svane, S., Sigurdarson, J.J., Sørensen, M.K., Hansen, M.J., Karring, H., & Feilberg, A. (2020). Synergistic tannic acid-fluoride inhibition of ammonia emissions and simultaneous reduction of methane and emissions from livestock waste. Environmental Science and Technology, 54(12), 7639–7650. https://doi.org/10.1021/acs.est.0c01231 DOI: https://doi.org/10.1021/acs.est.0c01231
Elihasridas, E., Pazla, R., Jamarun, N., Yanti, G., Sari, R.W.W., & Ikhlas, Z. (2023). Pre-treatments of Sonneratia alba fruit as the potential feed for ruminants using Aspergillus niger at different fermentation times: tannin concentration, enzyme activity, and total colonies. International Journal of Veterinary Science, 12(5), 755–761. https://doi.org/10.47278/journal.ijvs/2023.021 DOI: https://doi.org/10.47278/journal.ijvs/2023.021
Elihasridas, E., Pazla, R., Jamarun, N., Yanti, G., Asmairicen, S., Marlina, L., Hadiatry, M.C., Arief, R.W., Bansi, H., Khan, S.U., Khan, F.A., Putri, E.M., Antonius, A., Ikhlas, Z., Siva, A.T., Yendrita, H., & Zelinea, F. (2024). Effect of tannin degradation of mangrove fruit (Sonneratia alba) on nutrient digestibility, protozoa population, and methane gas production. Czech Journal of Animal Science, 69(7), 292–301. https://doi.org/10.17221/38/2024-CJAS DOI: https://doi.org/10.17221/38/2024-CJAS
Elihasridas, E., Pazla, R., Zain, M., Antonius, A., Ikhlas, Z., & Yanti, G. (2025). Optimizing mangrove-derived tannin extract for sustainable ruminant feeding: A strategy to balance nutrient digestibility and methane mitigation. Journal of Animal Behaviour and Biometeorology, 13(2), e2025012. https://doi.org/10.31893/jabb.2025012 DOI: https://doi.org/10.31893/jabb.2025012
Getachew, G., Pittroff, W., Putnam, D., Dandekar, A., Goyal, S., & DePeters, E.J. (2008). The influence of addition of gallic acid, tannic acid or quebracho tannins to alfalfa hay on in vitro rumen fermentation and microbial protein synthesis. Animal Feed Science and Technology, 140(3-4), 444–461. https://doi.org/10.1016/j.anifeedsci.2007.03.011 DOI: https://doi.org/10.1016/j.anifeedsci.2007.03.011
Guerreiro, O., Alves, S.P., Costa, M., Duarte, M.F., Jerónimo, E., & Bessa, R.J.B. (2021). Effects of increasing doses of condensed tannins from Cistus ladanifer L. on in vitro ruminal fermentation and biohydrogenation. Animals, 11(3), 761. https://www.mdpi.com/2076-2615/11/3/761# DOI: https://doi.org/10.3390/ani11030761
Jamarun, N., Pazla, R., Jayanegara, A., Elihasridas, E., Yanti, G., Antonius, A., Ikhlas,Z., & Ardani, L.R. (2025). Evaluation of lime treatment in reducing tannin content and enhancing nutrient utilization of mangrove fruits (Sonneratia alba) as an animal feed ingredient. Emirates Journal of Food and Agriculture, 37, 1–11. https://doi.org/10.3897/ejfa.2025.126901 DOI: https://doi.org/10.3897/ejfa.2025.126901
Min, B. R., & Solaiman, S. (2018). Comparative aspects of plant tannins on digestive physiology, nutrition and microbial community changes sheep and goats: A review. Journal of Animal Physiology and Animal Nutrition, 102(5), 1181–1193. https://doi.org/10.1111/jpn.12938 DOI: https://doi.org/10.1111/jpn.12938
Pazla, R., Antonius, A., Ikhlas, Z., Jayanegara, A., Ardani, L.R., Hadiatry, M.C., Jumjunidang, J., Budiyanti, T., Firsoni, F., Ardinal, A., Iswari, K., Febrina, D., & Ratni, E. (2025a). The potential of tannin from Sonneratia alba fruit in defaunation of rumen protozoa and reducing methane gas production: An approach to increase livestock digestive efficiency. Czech Journal of Animal Science, 70(6), 223–233. https://doi.org/10.17221/130/2024-CJAS DOI: https://doi.org/10.17221/130/2024-CJAS
Pazla, R., Antonius, A., Jamarun, N., Elihasridas, E., Ikhlas, Z., Fitri, Y., Rohaeni, E.S., Usman, U., Rostiana, O., Waryat, W., Setyadjit, S., Lintang, M., Yani, A., Joseph, G.H., Marlina, L., Ardinal, A. (2025b). Encapsulation gambir catechin-palm fatty acid distillate as an antibacterial and antioxidant feed additive and its effects on rumen fermentation products. Results in Engineering, 27, 105914. https://doi.org/10.1016/j.rineng.2025.105914 DOI: https://doi.org/10.1016/j.rineng.2025.105914
Tilley, J.M.A., & Terry. R.A. (1963). A two stage technique for in the in vitro digestion of forage crops. Grass and Forage Science, 18(2), 104-111. https://doi.org/10.1111/j.1365-2494.1963.tb00335.x DOI: https://doi.org/10.1111/j.1365-2494.1963.tb00335.x
Van Soest, P.J., Robertson, J.B., & Lewis, B.A. (1991). Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74(10), 3583–3597. https://doi.org/10.3168/jds.S0022-0302(91)78551-2 DOI: https://doi.org/10.3168/jds.S0022-0302(91)78551-2
Vasta, V., Mele, M., & Serra, A. (2009). Ruminal biohydrogenation as affected by tannins in vitro. The British Journal of Nutrition, 102(1), 82–92. https://doi.org/10.1016/j.anifeedsci.2019.114323 DOI: https://doi.org/10.1017/S0007114508137898
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Copyright (c) 2025 Elihasridas, Roni Pazla, Antonius, Gusri Yanti, Zaitul Ikhlas, Yelly Fitri, Jenni Zulfadillah, Meiko Armanda Bariva, Muhammad Arief Subhi, Natasya Triana, Todif Elfindo
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