Tannin and Saponin Contents of Different Organs of Melastoma Malabathricum L. as a Preliminary Basis for Methane-Mitigation Research in Ruminants
DOI:
https://doi.org/10.25077/alive.v3.n1.p82-87.2026Keywords:
Melastoma malabathricum L., methane mitigation, phytogenic additive, ruminants, saponin, senduduk, tanninAbstract
Melastoma (M) malabathricum L. is a phytochemically rich tropical plant with potential relevance to methane-mitigation research in ruminants. This study aimed to characterize the tannin and saponin contents of different organs of M. malabathricum L. as a preliminary basis for evaluating its prospective use as a phytogenic resource for livestock. Samples were collected from Padang Pariaman Regency, West Sumatra, Indonesia, and separated into roots, stems, leaves, flowers, and fruits for analysis. Tannin content was analyzed using the hide powder method based on the Folin-Ciocalteu reaction, while saponin content was determined using the TLC scanner method. The results showed clear variations in both metabolites among plant organs. Flowers had the highest tannin content, whereas the roots had the highest saponin content. These findings indicate an organ-specific distribution of bioactive compounds in M. melabathricum L. and suggest that this plant may serve as a promising botanical resource for further studies on methane mitigation. However, as the present study was limited to phytochemical characterization, further in vitro and in vivo studies are required to verify the effects of this extract on rumen fermentation and methane production.
Downloads
References
Antonius, A., Pazla, R., Putri, E. M., Alma’i, M. I., Laconi, E. B., Diapari, D., ... & Negoro, P. S. (2024). Effects of herbal plant supplementation on rumen fermentation profiles and protozoan population in vitro. Veterinary World, 17(5), 1139–1148. https://doi.org/10.14202/vetworld.2024.1139-1148
Antonius, A., Pazla, R., Putri, E. M., Negara, W., Laia, N., Ridla, M., Suharti, S., Jayanegara, A., Asmairicen, S., Marlina, L., & Marta, Y. (2023). Effectiveness of herbal plants on rumen fermentation, methane gas emissions, in vitro nutrient digestibility, and protozoa population. Veterinary World, 16(8), 1477–1488. https://doi.org/10.14202/vetworld.2023.1477-1488
Fonseca, N. V. B., Oliveira, S. G., Corrêa, A. M. R., Souza, T. T., Silva, G. G. A., Silva, E. C., & Ferreira, A. C. H. (2023). Additive tannins in ruminant nutrition: An alternative to antibiotics? Sustainability, 15(5), 4162. https://doi.org/10.3390/su15054162
Haroun, M., & Ahmed, M. (2022). Hide power and combined methods for the characterization of vegetable tannin in plants. GSC Advanced Research and Reviews, 13(3), 97–102. https://doi.org/10.30574/gscarr.2022.13.3.0348
Jayanegara, A., Yogianto, Y., Wina, E., Sudarman, A., Kondo, M., Obitsu, T., & Kreuzer, M. (2020). Combination effects of plant extracts rich in tannins and saponins as feed additives for mitigating in vitro ruminal methane and ammonia formation. Animals, 10(9), 1531. https://doi.org/10.3390/ani10091531
Joffry, S. M., Yob, N. J., Rofiee, M. S., Affandi, M. M. R. M. M., Suhaili, Z., Othman, F., Akim, A. M., Desa, M. N. M., & Zakaria, Z. A. (2012). Melastoma malabathricum (L.) Smith ethnomedicinal uses, chemical constituents, and pharmacological properties: A review. Evidence-Based Complementary and Alternative Medicine, 2012, 258434. https://doi.org/10.1155/2012/258434
Ku-Vera, J. C., Jiménez-Ocampo, R., Valencia-Salazar, S. S., Montoya-Flores, M. D., Molina-Botero, I. C., Arango, J., Gómez-Bravo, C. A., Aguilar-Pérez, C. F., & Solorio-Sánchez, F. J. (2020). Role of secondary plant metabolites on enteric methane mitigation in ruminants. Frontiers in Veterinary Science, 7, 584. https://doi.org/10.3389/fvets.2020.00584
Laura, E., Zain, M., & Agustin, F. (2024). Enhancing Sheep Rations: Optimal Ammoniated Palm Fronds Utilization with Lerak Fruit (Sapindus Rarak) and Probiotic Supplementation. Andalasian Livestock, 1(1), 59–69. https://doi.org/10.25077/alive.v1.n1.p59-69.2024
Patra, A. K., & Saxena, J. (2010). A new perspective on the use of plant secondary metabolites to inhibit methanogenesis in the rumen. Phytochemistry, 71(11–12), 1198–1222. https://doi.org/10.1016/j.phytochem.2010.05.010
Pazla, R., Zain, M., Antonius, A., Natsir, A., Hidayat, R., … Utami, B. V. (2025). In Vitro Evaluation of Gambier Leaf Extract (Uncaria gambir Roxb.) from Pangkalan, West Sumatra, as a Natural Feed Additive to Improve Nutrient Digestibility in Ruminants. Andalasian Livestock, 2(2), 170–177. https://doi.org/10.25077/alive.v2.n2.p170-177.2025
Pazla, R., Antonius, A., Mohd-Aris, A., Ikhlas, Z., Fitri, Y., Bansi, H., Dewi, Y. L., Hadiatry, M. C., Qomariyah, N., Wahyuwardani, S., Yusriani, Y., Rohaeni, E. S., & Bakrie, B. (2026). The role of tannin-based products in mitigating enteric methane emissions in ruminant livestock: A review. Journal of Advanced Veterinary and Animal Research, 13(1), 192–206. https://doi.org/10.5455/javar.2026.m1023
Ramaiyulis, R., Metri, Y., Irda, I., Kurnia, D., & Syukriani, D. (2022). Effects of Tannin-containing Supplement on Enteric Methane Emissions, Total Digestible Nutrient, and Average Daily Gain of Local Indonesian Beef Cattle. World Veterinary Journal, 12(4), 358–362. https://dx.doi.org/10.54203/scil.2022.wvj45
Tseten, T., Sanjorjo, R. A., Kwon, M., & Kim, S.-W. (2022). Strategies to Mitigate Enteric Methane Emissions from Ruminant Animals. Journal of Microbiology and Biotechnology, 32(3), 269–277. https://doi.org/10.4014/jmb.2202.02019
Widowati, R., Handayani, S., & Al Fikri, A. R. (2021). Phytochemical Screening and Antibacterial Activities of Senggani (Melastoma malabathricum L.) Ethanolic Extract Leaves. Jurnal Ilmu Pertanian Indonesia, 26(4), 562–568. https://doi.org/10.18343/jipi.26.4.56
Zheng, W.-J., Ren, Y.-S., Wu, M.-L., Yang, Y.-L., Fan, Y., Piao, X.-H., Ge, Y.-W., & Wang, S.-M. (2021). A review of the traditional uses, phytochemistry, and biological activities of the Melastoma genus. Journal of Ethnopharmacology, 264, 113322. https://doi.org/10.1016/j.jep.2020.113322
Downloads
Published
How to Cite
Issue
Section
License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

